Journal: International Journal of Molecular Sciences

Article Title: Development of a Raman-Based Method for the Diagnosis of People with Obstructive Sleep Apnea Syndrome: The Role of Lactic Acid

doi: 10.3390/ijms26189095

Figure Lengend Snippet: Box plots showing the distributions of the concentrations of cortisol ( a ), Superoxide Dismutase 3 (SOD3) ( b ), and lactate ( c ) obtained using Enzyme-Linked ImmunoSorbent Assay (ELISA) and colorimetric assays on the salivary samples of OSAS and CTR subjects. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: For SOD3 quantification, an ELISA kit already validated for salivary samples (CUSABIO ® , Biotech Co., Ltd., Wuhan, China) (detection sensitivity of 1.95 pg/mL) was also used; the kit had a detection range of 7.8 pg/mL–500 pg/mL.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Antioxidants

Article Title: Vitamin D Protects Pancreatic Cancer (PC) Cells from Death and DNA Damage Induced by Oxidative Stress

doi: 10.3390/antiox14091101

Figure Lengend Snippet: The effect of vitamin D 3 (Vit. D 3 ), hydrogen peroxide (H 2 O 2 ), N-Acetyl-L-Cysteine (NAC), the combination of Vit. D 3 with H 2 O 2 (Vit. D 3 + H 2 O 2 ) and the combination of NAC with H 2 O 2 (NAC + H 2 O 2 ) on mRNA expression of the antioxidant enzymes ( a ) catalase (CAT), ( b ) sodium dismutase 1 (SOD1), ( c ) sodium dismutase 2 (SOD2), ( d ) sodium dismutase 3 (SOD3) and ( e ) glutathione peroxidase 3 (Gpx3) in 1.2B4 cells. The 1.2B4 cells were non-treated (C, black bars) and treated with Vit D 3 (75 nM; 100 nM, yellow bars), H 2 O 2 (400 µM, brown bars), Vit. D 3 (75 nM; 100 nM) with H 2 O 2 (400 µM, orange bars), NAC (3 mM, blue bars), NAC (3 mM) with H 2 O 2 (400 µM, violet bars) for 24, 48 and 72 h. Data are expressed as the mean ± standard deviation (SD) of the fold change of three independent experiments in relation to the untreated control. GAPDH was used as a reference gene. a p < 0.05; aa p < 0.01; aaa p < 0.001 vs. C; b p < 0.05; bb p < 0.01; bbb p < 0.001 vs. H 2 O 2 ; c p < 0.05; cc p < 0.01 vs. 75D 3 ; d p < 0.05; dd p < 0.01 vs. 100D 3 ; e p < 0.05; ee p < 0.01 vs. 75D 3 + H 2 O 2 ; f p < 0.05 vs. 100D 3 + H 2 O 2 .

Article Snippet: The reaction of reverse transcription was conducted using a High Capacity cDNA Reverse Transcription Kit (Thermofisher Scientific Inc., Waltham, MA, USA), according to the manufacturer’s protocol. cDNA was employed to run qRT-PCR by using the TaqMan assays targeting the studied genes (ID: Hs00156308_m1 for CAT, Hs00533490_m1 for SOD1, Hs00167309_m1 for SOD2, Hs00162090_m1 for SOD3, Hs00173566_m1 for Gpx3, Hs99999905_m1 for GAPDH) (Life Technologies, Carlsbad, CA, USA) and TaqMan Universal Master Mix (Life Technologies, Carlsbad, CA, USA).

Techniques: Expressing, Standard Deviation, Control

Journal: Antioxidants

Article Title: Vitamin D Protects Pancreatic Cancer (PC) Cells from Death and DNA Damage Induced by Oxidative Stress

doi: 10.3390/antiox14091101

Figure Lengend Snippet: The effect of vitamin D 3 (Vit. D 3 ), hydrogen peroxide (H 2 O 2 ), N-Acetyl-L-Cysteine (NAC), the combination of Vit. D 3 with H 2 O 2 (Vit. D 3 + H 2 O 2 ) and the combination of NAC with H 2 O 2 (NAC + H 2 O 2 ) on the mRNA expression of the antioxidant enzymes ( a ) catalase (CAT), ( b ) sodium dismutase 1 (SOD1), ( c ) sodium dismutase 2 (SOD2), ( d ) sodium dismutase 3 (SOD3), ( e ) glutathione peroxidase 3 (Gpx3) in PANC-1 cells. The PANC-1 cells were non-treated (C, black bars) and treated with Vit D 3 (75 nM; 100 nM, yellow bars), H 2 O 2 (300 µM, brown bars), Vit. D 3 (75 nM; 100 nM) with H 2 O 2 (300 µM, orange bars), NAC (3 mM, blue bars) and NAC (3 mM) with H 2 O 2 (300, violet bars) for 24, 48 and 72 h. Data are expressed as the mean ± standard deviation (SD) of the fold change of three independent experiments in relation to the untreated control. GAPDH was used as a reference gene. a p < 0.05; aa p < 0.01; aaa p < 0.001 vs. C; b p < 0.05; bb p < 0.01; bbb p < 0.001 vs. H 2 O 2 ; c p < 0.05; cc p < 0.01 vs. 75D 3 ; d p < 0.05; dd p < 0.01; ddd p < 0.001 vs. 100D 3 ; e p < 0.05; ee p < 0.01 vs. 75D 3 + H 2 O 2 ; f p < 0.05; ff p < 0.01 vs. 100D 3 + H 2 O 2 ; g p < 0.05; gg p < 0.01 vs. NAC.

Article Snippet: The reaction of reverse transcription was conducted using a High Capacity cDNA Reverse Transcription Kit (Thermofisher Scientific Inc., Waltham, MA, USA), according to the manufacturer’s protocol. cDNA was employed to run qRT-PCR by using the TaqMan assays targeting the studied genes (ID: Hs00156308_m1 for CAT, Hs00533490_m1 for SOD1, Hs00167309_m1 for SOD2, Hs00162090_m1 for SOD3, Hs00173566_m1 for Gpx3, Hs99999905_m1 for GAPDH) (Life Technologies, Carlsbad, CA, USA) and TaqMan Universal Master Mix (Life Technologies, Carlsbad, CA, USA).

Techniques: Expressing, Standard Deviation, Control